Generation of Soybean (Glycine max) Transient Transgenic Roots
نویسندگان
چکیده
منابع مشابه
Soybean [Glycine max (L.) Merr].
In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wound...
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Studies were conducted with 9 to 12 day-old soybean (Glycine max [L.] Merr. cv. Williams) seedlings to determine the contribution of roots to whole plant NO(3) (-) reduction. Using an in vivo -NO(3) (-) nitrate reductase (NR) assay (no exogenous NO(3) (-) added to incubation medium) developed for roots, the roots accounted for approximately 30% of whole plant nitrate reductase activity (NRA) of...
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This research intended to analyze the expression pattern of proteins in roots of the Brazilian soybean cultivar Conquista when inoculated with Bradyrhizobium japonicum CPAC 15, a strain broadly used in commercial inoculants in Brazil. At ten days after bacterial inoculation, whole-cell proteins were extracted from roots and separated by 2-D gel electrophoresis. Comparative analysis revealed sig...
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Using precise pollen species determination by conventional microscopic methods, accompanied by molecular genetic markers, we found bees collect GMO (genetically modified) soybean pollen and incorporate it in Yucatan honey. Honey comb samples from Las Flores, Campeche, Mexico, often contained soybean pollen. Pollen in honey was analyzed in nine samples; six contained substantial soy pollen and t...
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and to be colonized by arbuscular mycorrhiza (AM) fungi (Harrison, 1997). Often, nodule formation by a Isoforms of endochitinase in soybean were studied in given bacterial strain is restricted to only a few host plant relation to root symbiosis. Five selected cultivars difgenera, and varieties of a given legume species may differ fering in their nodulation potential were inoculated in nodule fo...
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ژورنال
عنوان ژورنال: Current Protocols in Plant Biology
سال: 2016
ISSN: 2379-8068,2379-8068
DOI: 10.1002/cppb.20017